Detailed information of TA system TA288261

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67436..67862
• Replicon: plasmid 2
• Accession: NZ_LR213453
• Organism: Shigella flexneri strain AUSMDU00008355 isolate AUSMDU00008355

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: E0E33_RS23310
• Protein ID: WP_001312861.1
• Coordinates: 67436..67594 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67638..67862 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0E33_RS23270	62504..62743	-	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-
E0E33_RS23275	62851..63498	-	648	WP_128867105.1	transcriptional regulator TraJ family protein	-
E0E33_RS23280	63690..64073	-	384	WP_001151529.1	relaxosome protein TraM	-
E0E33_RS23285	64406..64996	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
E0E33_RS23290	65293..66114	-	822	WP_001234445.1	DUF945 domain-containing protein	-
E0E33_RS23295	66225..66521	-	297	WP_001272251.1	hypothetical protein	-
E0E33_RS23305	66821..67198	+	378	Protein_84	hypothetical protein	-
E0E33_RS23310	67436..67594	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	67638..67862	-	225	NuclAT_0	-	Antitoxin
-	67638..67862	-	225	NuclAT_0	-	Antitoxin
-	67638..67862	-	225	NuclAT_0	-	Antitoxin
-	67638..67862	-	225	NuclAT_0	-	Antitoxin
E0E33_RS23315	67674..67853	+	180	WP_001334662.1	hypothetical protein	-
E0E33_RS23320	67874..68593	-	720	WP_033807739.1	plasmid SOS inhibition protein A	-
E0E33_RS23325	68590..69024	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
E0E33_RS23330	69079..71037	-	1959	WP_112324844.1	ParB/RepB/Spo0J family partition protein	-
E0E33_RS23335	71101..71334	-	234	WP_000005971.1	DUF905 domain-containing protein	-
E0E33_RS23340	71390..71869	-	480	WP_130862260.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / sul1 / qacE / aadA5 / blaTEM-1B	-	1..82887	82887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T288261 WP_001312861.1 NZ_LR213453:c67594-67436 [Shigella flexneri]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT288261 NZ_LR213453:c67862-67638 [Shigella flexneri]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin