Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3472534..3473228 Replicon chromosome
Accession NZ_LR213452
Organism Shigella flexneri strain AUSMDU00008355 isolate AUSMDU00008355

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag E0E33_RS18090 Protein ID WP_001263491.1
Coordinates 3472830..3473228 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag E0E33_RS18085 Protein ID WP_000554759.1
Coordinates 3472534..3472827 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0E33_RS18065 3468312..3468833 + 522 Protein_3438 C40 family peptidase -
E0E33_RS18070 3468887..3470599 - 1713 Protein_3439 flagellar biosynthesis protein FlhA -
E0E33_RS18075 3470571..3471356 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
E0E33_RS18080 3471427..3472482 + 1056 WP_001226172.1 DNA polymerase IV -
E0E33_RS18085 3472534..3472827 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
E0E33_RS18090 3472830..3473228 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
E0E33_RS18095 3473238..3473690 + 453 WP_001059846.1 GNAT family N-acetyltransferase -
E0E33_RS18100 3473936..3474979 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
E0E33_RS18105 3475041..3475655 + 615 Protein_3446 peptide chain release factor H -
E0E33_RS18110 3475712..3477169 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
E0E33_RS18115 3477431..3477889 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB 3466450..3513013 46563


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T288256 WP_001263491.1 NZ_LR213452:3472830-3473228 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT288256 WP_000554759.1 NZ_LR213452:3472534-3472827 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References