288255

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3391374..3392218 Replicon chromosome
Accession NZ_LR213452
Organism Shigella flexneri strain AUSMDU00008355 isolate AUSMDU00008355

Toxin (Protein)


Gene name itaT Uniprot ID A0A4P7TPN5
Locus tag E0E33_RS17675 Protein ID WP_005060796.1
Coordinates 3391820..3392218 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag E0E33_RS17665 Protein ID WP_005053053.1
Coordinates 3391374..3391685 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0E33_RS17640 3386844..3387341 + 498 Protein_3352 COX aromatic rich motif-containing protein -
E0E33_RS17645 3387363..3389354 + 1992 Protein_3353 cytochrome o ubiquinol oxidase subunit I -
E0E33_RS17650 3389344..3389958 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
E0E33_RS17655 3389958..3390287 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
E0E33_RS17660 3390299..3391189 + 891 WP_000971356.1 heme o synthase -
E0E33_RS17665 3391374..3391685 + 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
E0E33_RS17670 3391669..3391827 + 159 WP_005053055.1 hypothetical protein -
E0E33_RS17675 3391820..3392218 + 399 WP_005060796.1 GNAT family N-acetyltransferase Toxin
E0E33_RS17680 3392266..3392963 - 698 Protein_3360 IS1 family transposase -
E0E33_RS17685 3393114..3395267 + 2154 WP_005060811.1 SEL1-like repeat protein -
E0E33_RS17690 3395320..3397050 + 1731 WP_000645011.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3392266..3392769 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(52-112)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14335.69 Da        Isoelectric Point: 9.1674

>T288255 WP_005060796.1 NZ_LR213452:3391820-3392218 [Shigella flexneri]
VRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKK
IHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 399 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT288255 WP_005053053.1 NZ_LR213452:3391374-3391685 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPN5

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References