Detailed information of TA system TA028809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13572..14229
• Replicon: plasmid pEA1509_A
• Accession: NC_020180
• Organism: Enterobacter aerogenes EA1509E

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EAEROS_RS00175
• Protein ID: WP_015365434.1
• Coordinates: 13879..14229 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EAEROS_RS00170
• Protein ID: WP_000124640.1
• Coordinates: 13572..13874 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAEROS_RS00120	9198..9626	+	429	WP_000591074.1	hypothetical protein	-
EAEROS_RS00125	9683..10042	+	360	WP_000422768.1	hypothetical protein	-
EAEROS_RS00130	10042..10488	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
EAEROS_RS00135	10485..11003	+	519	WP_000210758.1	nitrite reductase	-
EAEROS_RS00140	11003..11233	+	231	WP_000972663.1	hypothetical protein	-
EAEROS_RS00145	11220..12077	+	858	WP_001167032.1	hypothetical protein	-
EAEROS_RS00155	12308..12889	+	582	WP_015365433.1	nuclease	-
EAEROS_RS00160	12892..13164	+	273	WP_001043047.1	HU family DNA-binding protein	-
EAEROS_RS00165	13252..13545	+	294	WP_001239997.1	chromosome segregation protein ParM	-
EAEROS_RS00170	13572..13874	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
EAEROS_RS00175	13879..14229	-	351	WP_015365434.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EAEROS_RS00180	14392..14940	+	549	WP_001061574.1	transcriptional regulator	-
EAEROS_RS28025	15281..15475	+	195	WP_000343597.1	hypothetical protein	-
EAEROS_RS29085	15486..15859	+	374	Protein_24	hypothetical protein	-
EAEROS_RS00195	15852..16322	+	471	WP_001281821.1	hypothetical protein	-
EAEROS_RS00200	16337..16672	-	336	WP_000683476.1	hypothetical protein	-
EAEROS_RS00205	16769..17257	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
EAEROS_RS00210	17260..17757	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / blaTEM-24 / dfrA1 / ant(3'')-Ia / qacE / sul1	-	1..162202	162202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13314.19 Da
• Isoelectric Point: 5.2421

>T28809 WP_015365434.1 NC_020180:c14229-13879 [Klebsiella aerogenes EA1509E]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKEVRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T28809 NC_020180:c14229-13879 [Klebsiella aerogenes EA1509E]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGGTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT28809 WP_000124640.1 NC_020180:c13874-13572 [Klebsiella aerogenes EA1509E]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT28809 NC_020180:c13874-13572 [Klebsiella aerogenes EA1509E]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA