Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2840591..2841093 Replicon chromosome
Accession NC_020163
Organism Escherichia coli APEC O78

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag APECO78_RS14405 Protein ID WP_000767829.1
Coordinates 2840591..2840845 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag APECO78_RS14410 Protein ID WP_001259255.1
Coordinates 2840842..2841093 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO78_RS14380 2835607..2835834 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
APECO78_RS14385 2835848..2836906 - 1059 WP_000492346.1 transport protein YeeE -
APECO78_RS14390 2837084..2838442 - 1359 WP_015364500.1 putrescine/proton symporter PlaP -
APECO78_RS26760 2838432..2838494 - 63 WP_010723108.1 membrane protein YoeI -
APECO78_RS14395 2838709..2839638 - 930 WP_000803351.1 LysR family transcriptional regulator -
APECO78_RS14400 2839684..2840508 - 825 WP_000754758.1 SDR family oxidoreductase -
APECO78_RS14405 2840591..2840845 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
APECO78_RS14410 2840842..2841093 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
APECO78_RS25290 2841377..2841427 + 51 WP_001364200.1 his operon leader peptide -
APECO78_RS14415 2841573..2842472 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
APECO78_RS14420 2842478..2843782 + 1305 WP_001317939.1 histidinol dehydrogenase -
APECO78_RS14425 2843779..2844849 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
APECO78_RS14430 2844849..2845916 + 1068 WP_000080076.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T28801 WP_000767829.1 NC_020163:c2840845-2840591 [Escherichia coli APEC O78]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T28801 NC_020163:c2840845-2840591 [Escherichia coli APEC O78]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT28801 WP_001259255.1 NC_020163:c2841093-2840842 [Escherichia coli APEC O78]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT28801 NC_020163:c2841093-2840842 [Escherichia coli APEC O78]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References