Detailed information of TA system TA028779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3549443..3550113
• Replicon: chromosome
• Accession: NC_020089
• Organism: Mycobacterium tuberculosis 7199-99

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MT7199_RS16695
• Protein ID: WP_015345164.1
• Coordinates: 3549443..3549787 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: MT7199_RS16700
• Protein ID: WP_003899955.1
• Coordinates: 3549784..3550113 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT7199_RS16665	3544516..3545376	+	861	WP_003416628.1	alpha/beta hydrolase	-
MT7199_RS16670	3545351..3545866	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
MT7199_RS16675	3546106..3546399	+	294	WP_003416635.1	hypothetical protein	-
MT7199_RS16680	3546687..3547976	+	1290	WP_003416640.1	ATP-binding protein	-
MT7199_RS16685	3548323..3548757	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
MT7199_RS16690	3548760..3549212	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MT7199_RS16695	3549443..3549787	+	345	WP_015345164.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MT7199_RS16700	3549784..3550113	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
MT7199_RS16705	3550651..3550998	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
MT7199_RS16710	3550995..3551615	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
MT7199_RS16715	3551775..3553040	-	1266	WP_003899958.1	hypothetical protein	-
MT7199_RS16720	3553208..3553417	+	210	WP_003416778.1	hypothetical protein	-
MT7199_RS16730	3553664..3554698	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12609.42 Da
• Isoelectric Point: 5.6908

>T28779 WP_015345164.1 NC_020089:3549443-3549787 [Mycobacterium tuberculosis 7199-99]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRCYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T28779 NC_020089:3549443-3549787 [Mycobacterium tuberculosis 7199-99]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGCTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT28779 WP_003899955.1 NC_020089:3549784-3550113 [Mycobacterium tuberculosis 7199-99]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT28779 NC_020089:3549784-3550113 [Mycobacterium tuberculosis 7199-99]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Antitoxin

• AlphaFold DB: G0THF6