Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1043819..1044546 Replicon chromosome
Accession NZ_LR134315
Organism Escherichia coli strain NCTC9064

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag EL184_RS05080 Protein ID WP_000547564.1
Coordinates 1043819..1044130 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EL184_RS05085 Protein ID WP_000126294.1
Coordinates 1044127..1044546 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL184_RS05050 1038961..1040670 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
EL184_RS05055 1040680..1041222 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
EL184_RS05060 1041222..1041989 + 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
EL184_RS05065 1041986..1042396 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
EL184_RS05070 1042389..1042859 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
EL184_RS05075 1042884..1043645 + 762 WP_001026445.1 hypothetical protein -
EL184_RS05080 1043819..1044130 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
EL184_RS05085 1044127..1044546 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
EL184_RS05090 1044661..1046085 - 1425 WP_000110319.1 6-phospho-beta-glucosidase AscB -
EL184_RS05095 1046094..1047551 - 1458 WP_001107862.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
EL184_RS05100 1047811..1048821 + 1011 WP_001343660.1 DNA-binding transcriptional regulator AscG -
EL184_RS05105 1048970..1049497 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T287640 WP_000547564.1 NZ_LR134315:1043819-1044130 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT287640 WP_000126294.1 NZ_LR134315:1044127-1044546 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References