28755

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2206157..2207025 Replicon chromosome
Accession NC_020089
Organism Mycobacterium tuberculosis 7199-99

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag MT7199_RS10200 Protein ID WP_010886136.1
Coordinates 2206157..2206534 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag MT7199_RS10205 Protein ID WP_003409886.1
Coordinates 2206576..2207025 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT7199_RS10150 2201946..2202398 - 453 WP_003899095.1 lipoprotein -
MT7199_RS10155 2202462..2202863 + 402 WP_003409869.1 hypothetical protein -
MT7199_RS10160 2202856..2203038 - 183 WP_003409870.1 hypothetical protein -
MT7199_RS10165 2203152..2203502 - 351 WP_003899096.1 hypothetical protein -
MT7199_RS10170 2203513..2204415 - 903 WP_003899097.1 hypothetical protein -
MT7199_RS10175 2204436..2204627 - 192 WP_003409876.1 hypothetical protein -
MT7199_RS10180 2204628..2204924 - 297 WP_003409877.1 hypothetical protein -
MT7199_RS10185 2205164..2205379 + 216 WP_003409878.1 antitoxin -
MT7199_RS10190 2205376..2205687 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
MT7199_RS22455 2205661..2206182 - 522 WP_003904745.1 hypothetical protein -
MT7199_RS10200 2206157..2206534 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
MT7199_RS10205 2206576..2207025 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MT7199_RS10210 2207022..2207567 + 546 WP_003899098.1 SecB-like chaperone -
MT7199_RS10215 2207456..2208070 - 615 WP_003901296.1 hypothetical protein -
MT7199_RS10220 2208119..2208415 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
MT7199_RS10225 2208412..2208663 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
MT7199_RS10230 2208650..2209144 + 495 WP_003899099.1 hypothetical protein -
MT7199_RS10235 2209304..2209711 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
MT7199_RS10240 2209715..2209987 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MT7199_RS10245 2210020..2211240 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T28755 WP_010886136.1 NC_020089:2206157-2206534 [Mycobacterium tuberculosis 7199-99]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T28755 NC_020089:2206157-2206534 [Mycobacterium tuberculosis 7199-99]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT28755 WP_003409886.1 NC_020089:2206576-2207025 [Mycobacterium tuberculosis 7199-99]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT28755 NC_020089:2206576-2207025 [Mycobacterium tuberculosis 7199-99]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
AlphaFold DB G0TLM0

References