Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 5156348..5157138 Replicon chromosome
Accession NZ_LR134299
Organism Pseudomonas putida strain NCTC13186

Toxin (Protein)


Gene name yhaV Uniprot ID Q88NE5
Locus tag EL224_RS24225 Protein ID WP_010952397.1
Coordinates 5156671..5157138 (+) Length 156 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID I7C311
Locus tag EL224_RS24220 Protein ID WP_014859917.1
Coordinates 5156348..5156674 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL224_RS24195 5151882..5152943 - 1062 WP_049587435.1 HlyD family secretion protein -
EL224_RS24200 5152972..5154513 - 1542 WP_010952401.1 MFS transporter -
EL224_RS24205 5154630..5155535 - 906 WP_010952400.1 LysR family transcriptional regulator -
EL224_RS24215 5155809..5156255 + 447 WP_010952399.1 universal stress protein -
EL224_RS24220 5156348..5156674 + 327 WP_014859917.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
EL224_RS24225 5156671..5157138 + 468 WP_010952397.1 type II toxin-antitoxin system YhaV family toxin Toxin
EL224_RS24230 5157211..5158071 - 861 WP_010952396.1 HlyD family secretion protein -
EL224_RS24235 5158082..5158282 - 201 WP_003251718.1 DUF1656 domain-containing protein -
EL224_RS24240 5158272..5160449 - 2178 WP_003251716.1 FUSC family protein -
EL224_RS24245 5160446..5161975 - 1530 WP_010952395.1 efflux transporter outer membrane subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(17-152)

Antitoxin

PrlF antitoxin

(8-106)


Sequences

Toxin        


Download         Length: 156 a.a.        Molecular weight: 17962.34 Da        Isoelectric Point: 9.7963

>T287510 WP_010952397.1 NZ_LR134299:5156671-5157138 [Pseudomonas putida]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTKRNTFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWINDESSKRAYESSDDAYKVFQKMLHSGNPPDDWDQLLQEAAAD

Download         Length: 468 bp

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11919.40 Da        Isoelectric Point: 4.3722

>AT287510 WP_014859917.1 NZ_LR134299:5156348-5156674 [Pseudomonas putida]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Download         Length: 327 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88NE5

Antitoxin

Source ID Structure
AlphaFold DB I7C311

References