287506

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 1967721..1968760 Replicon chromosome
Accession NZ_LR134299
Organism Pseudomonas putida strain NCTC13186

Toxin (Protein)


Gene name PP_4152 Uniprot ID Q88FE5
Locus tag EL224_RS09175 Protein ID WP_010954922.1
Coordinates 1967721..1968296 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2V4F8J0
Locus tag EL224_RS09180 Protein ID WP_049588231.1
Coordinates 1968293..1968760 (-) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL224_RS09165 1963350..1964573 + 1224 WP_010954925.1 FAD-dependent oxidoreductase -
EL224_RS09170 1964577..1967507 - 2931 WP_049588229.1 aminotransferase -
EL224_RS09175 1967721..1968296 - 576 WP_010954922.1 PIN domain-containing protein Toxin
EL224_RS09180 1968293..1968760 - 468 WP_049588231.1 helix-turn-helix domain-containing protein Antitoxin
EL224_RS09185 1968908..1970512 - 1605 WP_010954920.1 ABC transporter ATP-binding protein -
EL224_RS09190 1970514..1971533 - 1020 WP_010954919.1 ABC transporter permease -
EL224_RS09195 1971535..1972608 - 1074 WP_010954918.1 microcin C ABC transporter permease YejB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1964577..1988404 23827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21646.86 Da        Isoelectric Point: 5.5710

>T287506 WP_010954922.1 NZ_LR134299:c1968296-1967721 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFSKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHSVQQYLDRLLQQKLPETVKLLSAFKFLL

Download         Length: 576 bp

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17112.54 Da        Isoelectric Point: 6.3403

>AT287506 WP_049588231.1 NZ_LR134299:c1968760-1968293 [Pseudomonas putida]
MTTANLSSHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRAAIDALAAQAQELKMGYE

Download         Length: 468 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88FE5

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F8J0

References