Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1368161..1368762 Replicon chromosome
Accession NZ_LR134299
Organism Pseudomonas putida strain NCTC13186

Toxin (Protein)


Gene name higB Uniprot ID A0A2L1IAQ2
Locus tag EL224_RS06360 Protein ID WP_049587984.1
Coordinates 1368448..1368762 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2L1IAR7
Locus tag EL224_RS06355 Protein ID WP_049587986.1
Coordinates 1368161..1368448 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL224_RS06335 1363193..1364038 + 846 WP_003254799.1 hypothetical protein -
EL224_RS06340 1364035..1365753 + 1719 WP_010952348.1 hypothetical protein -
EL224_RS06345 1365808..1366557 + 750 WP_049587988.1 hypothetical protein -
EL224_RS06350 1366629..1367960 - 1332 WP_004576169.1 30S ribosomal protein S12 methylthiotransferase RimO -
EL224_RS06355 1368161..1368448 - 288 WP_049587986.1 helix-turn-helix domain-containing protein Antitoxin
EL224_RS06360 1368448..1368762 - 315 WP_049587984.1 hypothetical protein Toxin
EL224_RS06365 1369211..1371121 + 1911 WP_010952353.1 potassium transporter Kup -
EL224_RS06370 1371170..1372462 - 1293 WP_010952354.1 virulence factor family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-88)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11784.61 Da        Isoelectric Point: 9.9097

>T287504 WP_049587984.1 NZ_LR134299:c1368762-1368448 [Pseudomonas putida]
MIFIETPVFTSDLKEHLDDEEYRALQAYLAEHPEAGSLLEETGGLRKIRWAAKGKGKSGGVRVIYYHVTAAHQIRMILIY
RKGIVDTLTSSQKAQLRALNKGWK

Download         Length: 315 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10841.31 Da        Isoelectric Point: 7.4063

>AT287504 WP_049587986.1 NZ_LR134299:c1368448-1368161 [Pseudomonas putida]
MEKHLFDRLVESMTQMDEIDRGERQPSREFHVDALQVKKIRQATGLSQAAFAKRIDVAVGTLRNWEQGRREPEGPARALL
RAIHNDPEHVLAALS

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1IAQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1IAR7

References