Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3677935..3678476 Replicon chromosome
Accession NZ_LR134296
Organism Escherichia coli strain NCTC9041

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag EL151_RS18025 Protein ID WP_000615983.1
Coordinates 3678198..3678476 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag EL151_RS18020 Protein ID WP_000729703.1
Coordinates 3677935..3678195 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL151_RS18005 3673986..3676079 + 2094 WP_001140826.1 flagellar biosynthesis protein FlhA -
EL151_RS18010 3676303..3676800 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
EL151_RS18015 3676976..3677725 - 750 WP_000056849.1 C40 family peptidase -
EL151_RS18020 3677935..3678195 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EL151_RS18025 3678198..3678476 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EL151_RS18030 3678632..3679372 + 741 WP_001225679.1 murein L,D-transpeptidase -
EL151_RS18035 3679343..3680110 - 768 WP_000333380.1 class II glutamine amidotransferase -
EL151_RS18040 3680316..3680894 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / tssM / tssA / hcp1/tssD1 3676976..3694085 17109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T287498 WP_000615983.1 NZ_LR134296:3678198-3678476 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT287498 WP_000729703.1 NZ_LR134296:3677935-3678195 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References