Detailed information of TA system TA287426

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 363890..364408
• Replicon: chromosome
• Accession: NZ_LR134271
• Organism: Staphylococcus aureus strain NCTC7988

Toxin (Protein)

• Gene name: holin
• Uniprot ID: -
• Locus tag: EL099_RS01785
• Protein ID: WP_000448764.1
• Coordinates: 364106..364408 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	363890..364026 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL099_RS01745	358984..359274	+	291	WP_000179860.1	hypothetical protein	-
EL099_RS01750	359290..361200	+	1911	WP_000429563.1	hypothetical protein	-
EL099_RS01755	361200..362666	+	1467	WP_000067157.1	BppU family phage baseplate upper protein	-
EL099_RS01760	362666..363055	+	390	WP_001166604.1	DUF2977 domain-containing protein	-
EL099_RS01765	363048..363212	+	165	WP_000916020.1	XkdX family protein	-
EL099_RS01770	363258..363557	+	300	WP_000466773.1	DUF2951 family protein	-
EL099_RS14880	363709..363898	+	190	Protein_335	putative holin-like toxin	-
-	363890..364026	-	137	NuclAT_0	-	Antitoxin
-	363890..364026	-	137	NuclAT_0	-	Antitoxin
-	363890..364026	-	137	NuclAT_0	-	Antitoxin
-	363890..364026	-	137	NuclAT_0	-	Antitoxin
EL099_RS01780	363945..364055	-	111	WP_070003492.1	hypothetical protein	-
EL099_RS01785	364106..364408	+	303	WP_000448764.1	phage holin	Toxin
EL099_RS01790	364420..365874	+	1455	WP_000930278.1	N-acetylmuramoyl-L-alanine amidase	-
EL099_RS01800	367388..367885	+	498	WP_001803960.1	hypothetical protein	-
EL099_RS01810	368445..369272	-	828	WP_000136028.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	320737..365874	45137
	-	inside	Prophage	-	geh	316180..365874	49694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11230.99 Da
• Isoelectric Point: 9.6587

>T287426 WP_000448764.1 NZ_LR134271:364106-364408 [Staphylococcus aureus]
METKVITRYIVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTAYKDNPTSQEGRWANQKLKKYKAENKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

Antitoxin

Nucleotide

• Length: 137 bp

>AT287426 NZ_LR134271:c364026-363890 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGACCTCTGTAGTTAAAT
GAATTTATATAATCCTCTAACCATCGTACTCGTCAAAGTTAGCGATGGTTATTTTTT

Structures

Antitoxin