Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3514440..3515152 Replicon chromosome
Accession NZ_LR134239
Organism Escherichia coli strain NCTC9100

Toxin (Protein)


Gene name yafO Uniprot ID -
Locus tag EL124_RS17150 Protein ID WP_126510788.1
Coordinates 3514440..3514856 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EL124_RS17155 Protein ID WP_000554758.1
Coordinates 3514859..3515152 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3510045..3510125 - 81 NuclAT_11 - -
- 3510045..3510125 - 81 NuclAT_11 - -
- 3510045..3510125 - 81 NuclAT_11 - -
- 3510045..3510125 - 81 NuclAT_11 - -
EL124_RS17125 3510721..3511179 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
EL124_RS17130 3511440..3512897 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
EL124_RS17135 3512954..3513475 - 522 Protein_3334 peptide chain release factor H -
EL124_RS17140 3513471..3513677 - 207 Protein_3335 RtcB family protein -
EL124_RS17145 3513995..3514447 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
EL124_RS17150 3514440..3514856 - 417 WP_126510788.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EL124_RS17155 3514859..3515152 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EL124_RS17160 3515204..3516260 - 1057 Protein_3339 DNA polymerase IV -
EL124_RS17165 3516331..3517116 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
EL124_RS17170 3517088..3518800 + 1713 Protein_3341 flagellar biosynthesis protein FlhA -
EL124_RS17175 3519024..3519521 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 16283.73 Da        Isoelectric Point: 6.9792

>T287319 WP_126510788.1 NZ_LR134239:c3514856-3514440 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRFLNLYYE

Download         Length: 417 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT287319 WP_000554758.1 NZ_LR134239:c3515152-3514859 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References