Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1252153..1252880 Replicon chromosome
Accession NZ_LR134237
Organism Escherichia coli strain NCTC9022

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag EL138_RS06190 Protein ID WP_000547555.1
Coordinates 1252153..1252464 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag EL138_RS06195 Protein ID WP_000126293.1
Coordinates 1252461..1252880 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL138_RS06160 1247384..1249003 + 1620 Protein_1192 formate hydrogenlyase subunit HycE -
EL138_RS06165 1249013..1249555 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
EL138_RS06170 1249555..1250322 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
EL138_RS06175 1250319..1250729 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
EL138_RS06180 1250722..1251192 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
EL138_RS06185 1251235..1251990 + 756 WP_001305268.1 hypothetical protein -
EL138_RS06190 1252153..1252464 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
EL138_RS06195 1252461..1252880 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
EL138_RS06200 1252972..1253400 - 429 WP_000536064.1 hypothetical protein -
EL138_RS06210 1253785..1254312 + 528 WP_001078777.1 electron transport protein HydN -
EL138_RS06215 1254465..1256717 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T287286 WP_000547555.1 NZ_LR134237:1252153-1252464 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT287286 WP_000126293.1 NZ_LR134237:1252461-1252880 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References