Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3723254..3723795 Replicon chromosome
Accession NZ_LR134236
Organism Escherichia coli strain NCTC9008

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag EL127_RS18305 Protein ID WP_000615979.1
Coordinates 3723517..3723795 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EL127_RS18300 Protein ID WP_000729704.1
Coordinates 3723254..3723514 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL127_RS18280 3718937..3719722 - 786 WP_000207549.1 putative lateral flagellar export/assembly protein LafU -
EL127_RS18285 3719694..3721406 + 1713 Protein_3552 flagellar biosynthesis protein FlhA -
EL127_RS18290 3721622..3722119 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
EL127_RS18295 3722295..3723068 - 774 WP_001353765.1 C40 family peptidase -
EL127_RS18300 3723254..3723514 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EL127_RS18305 3723517..3723795 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EL127_RS18310 3723951..3724691 + 741 WP_001225679.1 murein L,D-transpeptidase -
EL127_RS18315 3724662..3725429 - 768 WP_000333379.1 class II glutamine amidotransferase -
EL127_RS18320 3725573..3726151 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fdeC / gmhA/lpcA 3682872..3738709 55837


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T287278 WP_000615979.1 NZ_LR134236:3723517-3723795 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT287278 WP_000729704.1 NZ_LR134236:3723254-3723514 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References