Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 72186..72910 Replicon chromosome
Accession NZ_LR134235
Organism Klebsiella variicola strain NCTC9668

Toxin (Protein)


Gene name higB Uniprot ID A0A2J4YLV3
Locus tag EL159_RS00435 Protein ID WP_023292165.1
Coordinates 72186..72497 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EL159_RS00440 Protein ID WP_072122726.1
Coordinates 72494..72910 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL159_RS00415 68195..69097 - 903 WP_060598839.1 LysR family transcriptional regulator -
EL159_RS00420 69380..70519 + 1140 WP_072122725.1 PLP-dependent aspartate aminotransferase family protein -
EL159_RS00425 70540..71469 + 930 WP_158236771.1 DMT family transporter -
EL159_RS00430 71583..71881 + 299 Protein_73 DDE-type integrase/transposase/recombinase -
EL159_RS00435 72186..72497 + 312 WP_023292165.1 type II toxin-antitoxin system HigB family toxin Toxin
EL159_RS00440 72494..72910 + 417 WP_072122726.1 helix-turn-helix domain-containing protein Antitoxin
EL159_RS00445 73727..74107 + 381 WP_077138875.1 hypothetical protein -
EL159_RS00450 74173..74520 + 348 WP_077138876.1 hypothetical protein -
EL159_RS00455 74612..74842 + 231 WP_023340951.1 hypothetical protein -
EL159_RS00460 74889..75722 + 834 WP_060598878.1 type I restriction-modification system subunit M -
EL159_RS00465 76347..76877 + 531 Protein_80 antirestriction protein -
EL159_RS00470 76915..77394 - 480 WP_060598877.1 transglycosylase SLT domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-133)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12497.32 Da        Isoelectric Point: 9.9242

>T287247 WP_023292165.1 NZ_LR134235:72186-72497 [Klebsiella variicola]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Download         Length: 312 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15468.55 Da        Isoelectric Point: 4.4711

>AT287247 WP_072122726.1 NZ_LR134235:72494-72910 [Klebsiella variicola]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDTWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4YLV3

Antitoxin

Source ID Structure

References