Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3385685..3386287 Replicon chromosome
Accession NZ_LR134234
Organism Escherichia coli strain NCTC8623

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag EL131_RS16700 Protein ID WP_000897305.1
Coordinates 3385685..3385996 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EL131_RS16705 Protein ID WP_000356397.1
Coordinates 3385997..3386287 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL131_RS16670 3380715..3381500 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
EL131_RS16675 3381599..3382198 + 600 WP_001295269.1 glucose-1-phosphatase -
EL131_RS16680 3382192..3383064 + 873 WP_000920762.1 virulence factor BrkB family protein -
EL131_RS16685 3383061..3383498 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
EL131_RS16690 3383543..3384484 + 942 WP_001343389.1 fatty acid biosynthesis protein FabY -
EL131_RS16695 3384548..3385456 - 909 WP_001299484.1 alpha/beta hydrolase -
EL131_RS16700 3385685..3385996 + 312 WP_000897305.1 hypothetical protein Toxin
EL131_RS16705 3385997..3386287 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
EL131_RS16710 3386892..3387110 + 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
EL131_RS16715 3387329..3387571 + 243 WP_001087409.1 hypothetical protein -
EL131_RS16720 3387901..3388830 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
EL131_RS16725 3388827..3389462 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
EL131_RS16730 3389459..3390361 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T287241 WP_000897305.1 NZ_LR134234:3385685-3385996 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT287241 WP_000356397.1 NZ_LR134234:3385997-3386287 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References