Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2578824..2579623 Replicon chromosome
Accession NZ_LR134234
Organism Escherichia coli strain NCTC8623

Toxin (Protein)


Gene name yhaV Uniprot ID A0A6H2GMC5
Locus tag EL131_RS12720 Protein ID WP_000347279.1
Coordinates 2579159..2579623 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EL131_RS12715 Protein ID WP_001307405.1
Coordinates 2578824..2579159 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL131_RS12700 2574609..2575379 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
EL131_RS12705 2575395..2576729 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
EL131_RS12710 2577104..2578675 + 1572 WP_001273741.1 galactarate dehydratase -
EL131_RS12715 2578824..2579159 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EL131_RS12720 2579159..2579623 + 465 WP_000347279.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EL131_RS12725 2579678..2580487 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
EL131_RS12730 2580736..2582016 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
EL131_RS12735 2582039..2582512 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EL131_RS12740 2582523..2583302 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
EL131_RS12745 2583292..2584170 + 879 WP_001298758.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
EL131_RS12750 2584188..2584622 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2566670..2579623 12953
- inside Genomic island - - 2568397..2579623 11226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17869.24 Da        Isoelectric Point: 9.4947

>T287239 WP_000347279.1 NZ_LR134234:2579159-2579623 [Escherichia coli]
MDFPQRVNGWVLYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT287239 WP_001307405.1 NZ_LR134234:2578824-2579159 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H2GMC5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References