Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3537282..3537976 Replicon chromosome
Accession NZ_LR134227
Organism Escherichia coli strain NCTC9102

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag EL093_RS17320 Protein ID WP_001263489.1
Coordinates 3537282..3537680 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EL093_RS17325 Protein ID WP_000554758.1
Coordinates 3537683..3537976 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3532870..3532950 - 81 NuclAT_9 - -
- 3532870..3532950 - 81 NuclAT_9 - -
- 3532870..3532950 - 81 NuclAT_9 - -
- 3532870..3532950 - 81 NuclAT_9 - -
EL093_RS17295 3533546..3534004 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
EL093_RS17300 3534265..3535722 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
EL093_RS17305 3535779..3536300 - 522 Protein_3360 peptide chain release factor H -
EL093_RS17310 3536296..3536502 - 207 Protein_3361 RtcB family protein -
EL093_RS17315 3536820..3537272 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
EL093_RS17320 3537282..3537680 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EL093_RS17325 3537683..3537976 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EL093_RS17330 3538028..3539083 - 1056 WP_001226164.1 DNA polymerase IV -
EL093_RS17335 3539154..3539939 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
EL093_RS17340 3539911..3541623 + 1713 Protein_3367 flagellar biosynthesis protein FlhA -
EL093_RS17345 3541847..3542344 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3537282..3553499 16217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T287178 WP_001263489.1 NZ_LR134227:c3537680-3537282 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT287178 WP_000554758.1 NZ_LR134227:c3537976-3537683 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References