Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3593058..3593752 Replicon chromosome
Accession NZ_LR134216
Organism Escherichia coli strain NCTC10973

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag EL103_RS17420 Protein ID WP_001263489.1
Coordinates 3593058..3593456 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EL103_RS17425 Protein ID WP_000554758.1
Coordinates 3593459..3593752 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3588646..3588726 - 81 NuclAT_11 - -
- 3588646..3588726 - 81 NuclAT_11 - -
- 3588646..3588726 - 81 NuclAT_11 - -
- 3588646..3588726 - 81 NuclAT_11 - -
EL103_RS17395 3589322..3589780 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
EL103_RS17400 3590041..3591498 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
EL103_RS17405 3591555..3592076 - 522 Protein_3384 peptide chain release factor H -
EL103_RS17410 3592072..3592278 - 207 Protein_3385 RtcB family protein -
EL103_RS17415 3592596..3593048 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
EL103_RS17420 3593058..3593456 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EL103_RS17425 3593459..3593752 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EL103_RS17430 3593804..3594859 - 1056 WP_001226164.1 DNA polymerase IV -
EL103_RS17435 3594930..3595853 - 924 WP_001232546.1 putative lateral flagellar export/assembly protein LafU -
EL103_RS17440 3595856..3596719 - 864 WP_001169518.1 flagellar motor stator protein MotA -
EL103_RS17445 3596732..3597448 - 717 WP_000938723.1 FliA/WhiG family RNA polymerase sigma factor -
EL103_RS17450 3597468..3597935 - 468 WP_000725257.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T287100 WP_001263489.1 NZ_LR134216:c3593456-3593058 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT287100 WP_000554758.1 NZ_LR134216:c3593752-3593459 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References