Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 646836..647563 Replicon chromosome
Accession NZ_LR134216
Organism Escherichia coli strain NCTC10973

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag EL103_RS03160 Protein ID WP_000550189.1
Coordinates 646836..647150 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EL103_RS03165 Protein ID WP_000560266.1
Coordinates 647147..647563 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL103_RS03140 642990..643976 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
EL103_RS03145 644055..644747 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
EL103_RS03150 644828..645331 - 504 WP_001326618.1 M48 family metallopeptidase -
EL103_RS03155 645416..646552 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
EL103_RS03160 646836..647150 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
EL103_RS03165 647147..647563 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
EL103_RS03170 647608..649626 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
EL103_RS03175 650052..652403 - 2352 WP_000695485.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T287088 WP_000550189.1 NZ_LR134216:646836-647150 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT287088 WP_000560266.1 NZ_LR134216:647147-647563 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References