Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4611614..4612216 Replicon chromosome
Accession NZ_LR134209
Organism Escherichia coli strain NCTC11476

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag EL080_RS22255 Protein ID WP_000897305.1
Coordinates 4611905..4612216 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EL080_RS22250 Protein ID WP_000356397.1
Coordinates 4611614..4611904 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL080_RS22225 4607540..4608442 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
EL080_RS22230 4608439..4609074 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
EL080_RS22235 4609071..4610000 + 930 WP_000027706.1 formate dehydrogenase accessory protein FdhE -
EL080_RS22240 4610330..4610572 - 243 WP_001087409.1 hypothetical protein -
EL080_RS22245 4610791..4611009 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
EL080_RS22250 4611614..4611904 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
EL080_RS22255 4611905..4612216 - 312 WP_000897305.1 hypothetical protein Toxin
EL080_RS22260 4612445..4613353 + 909 WP_001162704.1 alpha/beta hydrolase -
EL080_RS22265 4613417..4614358 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
EL080_RS22270 4614403..4614840 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
EL080_RS22275 4614837..4615709 - 873 WP_000920762.1 virulence factor BrkB family protein -
EL080_RS22280 4615703..4616302 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T287044 WP_000897305.1 NZ_LR134209:c4612216-4611905 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT287044 WP_000356397.1 NZ_LR134209:c4611904-4611614 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References