Detailed information of TA system TA286780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5087531..5088133
• Replicon: chromosome
• Accession: NZ_LR134092
• Organism: Escherichia coli strain NCTC10444

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EL041_RS24500
• Protein ID: WP_000897305.1
• Coordinates: 5087822..5088133 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EL041_RS24495
• Protein ID: WP_000356397.1
• Coordinates: 5087531..5087821 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL041_RS24465	5083478..5084380	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EL041_RS24470	5084377..5085012	+	636	WP_000829031.1	formate dehydrogenase cytochrome b556 subunit	-
EL041_RS24475	5085009..5085938	+	930	WP_000027691.1	formate dehydrogenase accessory protein FdhE	-
EL041_RS24480	5086268..5086510	-	243	WP_001086385.1	hypothetical protein	-
EL041_RS24485	5086729..5086947	-	219	WP_001313440.1	ribbon-helix-helix domain-containing protein	-
EL041_RS24495	5087531..5087821	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EL041_RS24500	5087822..5088133	-	312	WP_000897305.1	hypothetical protein	Toxin
EL041_RS24505	5088362..5089270	+	909	WP_023155176.1	alpha/beta hydrolase	-
EL041_RS24510	5089438..5091252	-	1815	WP_000226343.1	hypothetical protein	-
EL041_RS24515	5091663..5092604	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
EL041_RS24520	5092649..5093086	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T286780 WP_000897305.1 NZ_LR134092:c5088133-5087822 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T286780 NZ_LR134092:c5088133-5087822 [Escherichia coli]
ATACTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT286780 WP_000356397.1 NZ_LR134092:c5087821-5087531 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT286780 NZ_LR134092:c5087821-5087531 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7