Detailed information of TA system TA028678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3448079..3448688
• Replicon: chromosome
• Accession: NC_019940
• Organism: Thioflavicoccus mobilis 8321

Toxin (Protein)

• Gene name: higB
• Uniprot ID: L0H2L3
• Locus tag: THIMO_RS14955
• Protein ID: WP_015281957.1
• Coordinates: 3448353..3448688 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: L0GYH7
• Locus tag: THIMO_RS14950
• Protein ID: WP_015281956.1
• Coordinates: 3448079..3448369 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THIMO_RS14940	3445755..3447182	+	1428	WP_041604618.1	efflux transporter outer membrane subunit	-
THIMO_RS14945	3447224..3447853	-	630	Protein_3044	helix-turn-helix transcriptional regulator	-
THIMO_RS14950	3448079..3448369	-	291	WP_015281956.1	helix-turn-helix transcriptional regulator	Antitoxin
THIMO_RS14955	3448353..3448688	-	336	WP_015281957.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
THIMO_RS20105	3449507..3449671	+	165	WP_015281958.1	hypothetical protein	-
THIMO_RS14965	3450327..3452330	-	2004	WP_015281959.1	DNA helicase Rep	-
THIMO_RS14970	3452411..3452875	-	465	WP_015281960.1	DUF2269 domain-containing protein	-
THIMO_RS14975	3453099..3453326	-	228	WP_015281961.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-90)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12646.81 Da
• Isoelectric Point: 10.1078

>T28678 WP_015281957.1 NC_019940:c3448688-3448353 [Thioflavicoccus mobilis 8321]
VPYCIIYYSEAVQTAITSLPDTLAARYIVLTRRMLDFGPNLGEPHTKAFGNGLFELRLKGADGIARVFFCTQVGQRIVML
HCFVKKTNKTPVRERRLAEARMKEIHHADPR

Nucleotide

• Length: 336 bp

>T28678 NC_019940:c3448688-3448353 [Thioflavicoccus mobilis 8321]
GTGCCCTACTGCATTATCTACTATAGCGAGGCCGTCCAGACGGCGATCACGTCGTTGCCCGACACCCTGGCGGCACGCTA
TATCGTCTTGACTCGGCGCATGCTGGATTTTGGTCCGAATCTGGGCGAGCCACATACCAAGGCATTCGGTAACGGCTTGT
TCGAGCTACGTCTGAAAGGCGCCGATGGGATCGCGCGTGTCTTCTTCTGCACGCAGGTCGGACAGCGGATCGTGATGCTG
CACTGCTTTGTGAAGAAGACCAACAAGACACCCGTGCGCGAACGGCGTCTCGCCGAAGCGCGGATGAAGGAGATCCACCA
TGCAGACCCACGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10700.26 Da
• Isoelectric Point: 7.3606

>AT28678 WP_015281956.1 NC_019940:c3448369-3448079 [Thioflavicoccus mobilis 8321]
MQTHDELIETLIQRPGVQAEVERLEREEFGLLDLLLKARHEAGLSQAQVAERMGTHAPAVARLERALATGKHSPSVATLR
KYARACGKNLFVDLRD

Nucleotide

• Length: 291 bp

>AT28678 NC_019940:c3448369-3448079 [Thioflavicoccus mobilis 8321]
ATGCAGACCCACGATGAACTGATCGAGACGCTGATACAGCGTCCCGGTGTGCAAGCCGAGGTCGAGCGCCTGGAGCGGGA
GGAATTCGGCCTGCTCGACCTGTTACTGAAGGCACGTCACGAGGCGGGGCTTTCCCAGGCTCAGGTCGCCGAGCGCATGG
GGACTCACGCGCCCGCGGTTGCGCGCTTGGAGCGGGCGCTGGCGACGGGCAAGCACTCGCCGTCCGTTGCCACCTTGCGC
AAATATGCTCGCGCTTGCGGCAAGAATCTCTTCGTGGATCTCAGGGACTAG

Structures

Toxin

• AlphaFold DB: L0H2L3

Antitoxin

• AlphaFold DB: L0GYH7