Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4145071..4145612 Replicon chromosome
Accession NZ_LR134092
Organism Escherichia coli strain NCTC10444

Toxin (Protein)


Gene name yafQ Uniprot ID U9XF38
Locus tag EL041_RS20180 Protein ID WP_001695460.1
Coordinates 4145334..4145612 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EL041_RS20175 Protein ID WP_000729704.1
Coordinates 4145071..4145331 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL041_RS20155 4140653..4141438 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
EL041_RS20160 4141410..4143122 + 1713 Protein_3866 flagellar biosynthesis protein FlhA -
EL041_RS20165 4143338..4143835 - 498 WP_000006265.1 REP-associated tyrosine transposase RayT -
EL041_RS20170 4144112..4144861 - 750 WP_001695461.1 C40 family peptidase -
EL041_RS20175 4145071..4145331 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EL041_RS20180 4145334..4145612 + 279 WP_001695460.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EL041_RS20185 4145768..4146508 + 741 WP_001225679.1 murein L,D-transpeptidase -
EL041_RS20190 4146479..4147246 - 768 WP_000333379.1 class II glutamine amidotransferase -
EL041_RS20195 4147452..4148030 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4144112..4153833 9721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T286774 WP_001695460.1 NZ_LR134092:4145334-4145612 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYIMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT286774 WP_000729704.1 NZ_LR134092:4145071-4145331 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XF38

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References