Detailed information of TA system TA028675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1898201..1898794
• Replicon: chromosome
• Accession: NC_019940
• Organism: Thioflavicoccus mobilis 8321

Toxin (Protein)

• Gene name: graT
• Uniprot ID: L0GYX1
• Locus tag: THIMO_RS08205
• Protein ID: WP_015280635.1
• Coordinates: 1898516..1898794 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: THIMO_RS08200
• Protein ID: WP_015280634.1
• Coordinates: 1898201..1898503 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THIMO_RS08170	1893655..1894356	+	702	WP_041604262.1	orotidine-5'-phosphate decarboxylase	-
THIMO_RS08175	1894508..1895383	+	876	WP_015280631.1	UTP--glucose-1-phosphate uridylyltransferase GalU	-
THIMO_RS08180	1895406..1896089	+	684	WP_015280632.1	phosphate signaling complex protein PhoU	-
THIMO_RS19835	1896118..1896237	+	120	Protein_1669	nucleotidyltransferase domain-containing protein	-
THIMO_RS08190	1896626..1896940	+	315	Protein_1670	hypothetical protein	-
THIMO_RS18985	1897222..1897359	-	138	WP_015280633.1	DUF29 family protein	-
THIMO_RS08195	1897605..1897997	+	393	WP_041603580.1	hypothetical protein	-
THIMO_RS08200	1898201..1898503	-	303	WP_015280634.1	HigA family addiction module antidote protein	Antitoxin
THIMO_RS08205	1898516..1898794	-	279	WP_015280635.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
THIMO_RS08210	1899732..1903589	-	3858	WP_015280637.1	DUF3683 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (25-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-93)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10514.95 Da
• Isoelectric Point: 8.5135

>T28675 WP_015280635.1 NC_019940:c1898794-1898516 [Thioflavicoccus mobilis 8321]
MIRSFRCKETEKLFGGSCPARFRAFQAVAERKLAQLNNASTLDFLRSPPGNRLEALSGDRTGSYSIRINAQWRLCFVWRE
GDACDVEIVDYH

Nucleotide

• Length: 279 bp

>T28675 NC_019940:c1898794-1898516 [Thioflavicoccus mobilis 8321]
ATGATCCGCTCGTTCCGCTGCAAAGAAACCGAGAAGCTTTTTGGCGGCAGTTGCCCGGCGCGGTTCCGCGCCTTTCAGGC
CGTCGCCGAGCGGAAATTGGCGCAGCTCAACAACGCGTCTACCCTGGACTTTCTGCGCAGCCCTCCCGGCAATCGGCTGG
AGGCCCTGAGCGGGGACCGAACGGGCAGCTACAGCATTCGGATCAACGCTCAATGGCGGCTCTGCTTCGTCTGGCGCGAG
GGCGACGCCTGCGATGTAGAGATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11125.75 Da
• Isoelectric Point: 8.6398

>AT28675 WP_015280634.1 NC_019940:c1898503-1898201 [Thioflavicoccus mobilis 8321]
MTPFNGMRPVHPGEILREDFLAPLGMSVNALAQALRVPATRLHEIVKERRAVTPDTALRLARYFGGDAQSWLNLQTSYDL
KIAERDLGANIERDVSPRAA

Nucleotide

• Length: 303 bp

>AT28675 NC_019940:c1898503-1898201 [Thioflavicoccus mobilis 8321]
ATGACACCCTTCAACGGCATGCGCCCGGTCCATCCCGGTGAGATCCTCCGGGAAGACTTCCTGGCGCCGCTCGGCATGAG
CGTCAATGCGCTTGCCCAAGCACTGCGTGTGCCGGCAACGCGCCTACACGAGATCGTGAAGGAACGGCGCGCGGTGACGC
CGGATACCGCCTTGCGCCTTGCGCGCTATTTCGGCGGTGACGCGCAATCCTGGCTGAACCTTCAGACGAGCTACGACCTG
AAGATCGCCGAGCGCGATCTCGGAGCAAACATCGAGCGGGATGTGAGCCCACGCGCGGCCTGA

Structures

Toxin

• AlphaFold DB: L0GYX1