Detailed information of TA system TA028671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 961602..962428
• Replicon: chromosome
• Accession: NC_019936
• Organism: Pseudomonas stutzeri RCH2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: L0GFH6
• Locus tag: PSEST_RS04325
• Protein ID: WP_015275816.1
• Coordinates: 961925..962428 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: L0GI82
• Locus tag: PSEST_RS04320
• Protein ID: WP_015275815.1
• Coordinates: 961602..961928 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSEST_RS04300	956834..957745	+	912	WP_015275812.1	restriction endonuclease	-
PSEST_RS21670	958287..958493	-	207	WP_197445814.1	hypothetical protein	-
PSEST_RS04305	958562..959458	-	897	WP_015275813.1	LysR family transcriptional regulator	-
PSEST_RS04310	959599..960588	+	990	WP_041756905.1	zinc-dependent alcohol dehydrogenase family protein	-
PSEST_RS04315	960738..961049	+	312	Protein_865	transposase	-
PSEST_RS22060	961033..961500	+	468	WP_157372375.1	hypothetical protein	-
PSEST_RS04320	961602..961928	+	327	WP_015275815.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
PSEST_RS04325	961925..962428	+	504	WP_015275816.1	type II toxin-antitoxin system YhaV family toxin	Toxin
PSEST_RS04330	963073..963375	-	303	WP_007182542.1	antibiotic biosynthesis monooxygenase	-
PSEST_RS04335	963442..964455	+	1014	WP_015275817.1	helix-turn-helix domain-containing protein	-
PSEST_RS21675	964660..964854	+	195	Protein_871	transposase	-
PSEST_RS04340	964958..966293	-	1336	Protein_872	DUF3422 family protein	-
PSEST_RS04345	966479..966877	-	399	WP_003291554.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	960738..960977	239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (16-152)

Antitoxin

• PrlF antitoxin (8-106)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 19223.69 Da
• Isoelectric Point: 9.5348

>T28671 WP_015275816.1 NC_019936:961925-962428 [Pseudomonas stutzeri RCH2]
MSQDQGKPLVAHGWTVFAHPLFLDQLETLIQQVELSKQKDPVGYRKNNAAKRLAAITKLAFDVIPQDPSKPEYRQGGTFG
EEHKHWFRAKFFQQYRLFFRYHGPSKVIILAWVNDEDTKRAYESSDDAYKVFRKMLASGHPPDSWDKLLNEATQENARLN
SAATSVN

Nucleotide

• Length: 504 bp

>T28671 NC_019936:961925-962428 [Pseudomonas stutzeri RCH2]
ATGAGCCAAGACCAAGGTAAGCCACTGGTTGCTCATGGCTGGACAGTATTTGCGCACCCCTTGTTTCTGGATCAGCTCGA
AACCCTCATTCAGCAAGTTGAGCTCTCAAAACAAAAAGACCCCGTGGGTTATCGCAAGAATAATGCAGCCAAGCGGCTGG
CAGCCATCACCAAGCTGGCTTTTGACGTTATCCCACAAGACCCGAGCAAGCCCGAATACCGCCAAGGTGGCACATTTGGC
GAGGAACATAAGCACTGGTTCCGTGCCAAGTTTTTTCAGCAGTATCGGCTGTTCTTCCGGTACCACGGCCCAAGCAAGGT
AATCATCTTGGCGTGGGTTAATGACGAAGACACCAAGCGTGCCTATGAGAGCAGCGATGACGCCTACAAGGTGTTTCGAA
AAATGCTCGCAAGCGGACATCCACCTGACTCGTGGGATAAGCTGCTGAATGAAGCCACGCAGGAAAACGCACGATTAAAT
TCTGCCGCCACCTCGGTCAACTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12019.35 Da
• Isoelectric Point: 4.4621

>AT28671 WP_015275815.1 NC_019936:961602-961928 [Pseudomonas stutzeri RCH2]
MARALEVESTLTDRYQTTVPETVRRALQLNKRDKIHYSIRPSGEVVLSRAEPTEDDPAIGQFLSFLAHDIAANPQHLRAI
DSDLVARIHSLVDSNDIDLDAPLSADDE

Nucleotide

• Length: 327 bp

>AT28671 NC_019936:961602-961928 [Pseudomonas stutzeri RCH2]
ATGGCCAGAGCACTTGAAGTCGAATCCACCCTTACCGACCGCTATCAGACGACGGTACCAGAAACGGTTCGCCGCGCACT
TCAGCTAAACAAGCGCGACAAAATCCATTACTCGATTCGCCCCAGCGGTGAGGTCGTGCTGAGCCGTGCTGAGCCCACTG
AGGATGACCCGGCAATCGGCCAGTTTCTCAGCTTTCTTGCACATGACATCGCAGCGAACCCTCAGCACCTGCGAGCCATA
GATTCAGATCTCGTGGCACGCATTCATTCGCTGGTCGATAGCAACGATATAGATCTGGATGCCCCGCTGTCGGCAGACGA
TGAATGA

Structures

Toxin

• AlphaFold DB: L0GFH6

Antitoxin

• AlphaFold DB: A0A5R9QF20