Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4832176..4832778 Replicon chromosome
Accession NZ_LR134080
Organism Escherichia coli strain NCTC9082

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag EL023_RS23745 Protein ID WP_000897305.1
Coordinates 4832467..4832778 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EL023_RS23740 Protein ID WP_000356397.1
Coordinates 4832176..4832466 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL023_RS23710 4827723..4828358 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
EL023_RS23715 4828355..4829284 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
EL023_RS23725 4829683..4830663 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
EL023_RS23730 4830892..4831134 - 243 WP_001086388.1 hypothetical protein -
EL023_RS23735 4831353..4831571 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
EL023_RS23740 4832176..4832466 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
EL023_RS23745 4832467..4832778 - 312 WP_000897305.1 hypothetical protein Toxin
EL023_RS23750 4833007..4833915 + 909 WP_001162704.1 alpha/beta hydrolase -
EL023_RS23755 4833979..4834920 - 942 WP_001343389.1 fatty acid biosynthesis protein FabY -
EL023_RS23760 4834965..4835402 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
EL023_RS23765 4835399..4836271 - 873 WP_000920762.1 virulence factor BrkB family protein -
EL023_RS23770 4836265..4836864 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4829683..4830663 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T286668 WP_000897305.1 NZ_LR134080:c4832778-4832467 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT286668 WP_000356397.1 NZ_LR134080:c4832466-4832176 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References