Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4248582..4249123 Replicon chromosome
Accession NZ_LR134079
Organism Escherichia coli strain NCTC9112

Toxin (Protein)


Gene name yafQ Uniprot ID U9XF38
Locus tag EL053_RS21200 Protein ID WP_001695460.1
Coordinates 4248845..4249123 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EL053_RS21195 Protein ID WP_000729704.1
Coordinates 4248582..4248842 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL053_RS21175 4244164..4244949 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
EL053_RS21180 4244921..4246633 + 1713 Protein_4081 flagellar biosynthesis protein FlhA -
EL053_RS21185 4246849..4247346 - 498 WP_000006265.1 REP-associated tyrosine transposase RayT -
EL053_RS21190 4247623..4248372 - 750 WP_001695461.1 C40 family peptidase -
EL053_RS21195 4248582..4248842 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EL053_RS21200 4248845..4249123 + 279 WP_001695460.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EL053_RS21205 4249279..4250019 + 741 WP_001225679.1 murein L,D-transpeptidase -
EL053_RS21210 4249990..4250757 - 768 WP_000333379.1 class II glutamine amidotransferase -
EL053_RS21215 4250963..4251541 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4247623..4257374 9751


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T286641 WP_001695460.1 NZ_LR134079:4248845-4249123 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYIMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT286641 WP_000729704.1 NZ_LR134079:4248582-4248842 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XF38

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References