Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4133211..4133708 Replicon chromosome
Accession NZ_LR134075
Organism Escherichia coli strain NCTC9084

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EL019_RS20225 Protein ID WP_001173343.1
Coordinates 4133211..4133384 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EL019_RS20230 Protein ID WP_000398619.1
Coordinates 4133412..4133708 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL019_RS20215 4129881..4131338 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
EL019_RS20220 4131575..4133092 + 1518 WP_126297571.1 lysine--tRNA ligase -
EL019_RS20225 4133211..4133384 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EL019_RS20230 4133412..4133708 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EL019_RS20235 4133935..4134207 - 273 WP_000405651.1 N-acetyltransferase -
EL019_RS20240 4134219..4134449 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
EL019_RS20245 4134630..4136261 + 1632 WP_001216477.1 sensor histidine kinase -
EL019_RS20250 4136258..4136977 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4124916..4133708 8792


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T286623 WP_001173343.1 NZ_LR134075:c4133384-4133211 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT286623 WP_000398619.1 NZ_LR134075:c4133708-4133412 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References