Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3559359..3560053 Replicon chromosome
Accession NZ_LR134075
Organism Escherichia coli strain NCTC9084

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag EL019_RS17415 Protein ID WP_001263489.1
Coordinates 3559359..3559757 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EL019_RS17420 Protein ID WP_000554758.1
Coordinates 3559760..3560053 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3554947..3555027 - 81 NuclAT_11 - -
- 3554947..3555027 - 81 NuclAT_11 - -
- 3554947..3555027 - 81 NuclAT_11 - -
- 3554947..3555027 - 81 NuclAT_11 - -
EL019_RS17390 3555623..3556081 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
EL019_RS17395 3556342..3557799 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
EL019_RS17400 3557856..3558377 - 522 Protein_3368 peptide chain release factor H -
EL019_RS17405 3558373..3558579 - 207 Protein_3369 RtcB family protein -
EL019_RS17410 3558897..3559349 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
EL019_RS17415 3559359..3559757 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EL019_RS17420 3559760..3560053 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EL019_RS17425 3560105..3561160 - 1056 WP_001226164.1 DNA polymerase IV -
EL019_RS17430 3561231..3562154 - 924 WP_001232546.1 putative lateral flagellar export/assembly protein LafU -
EL019_RS17435 3562157..3563020 - 864 WP_001169518.1 flagellar motor stator protein MotA -
EL019_RS17440 3563033..3563749 - 717 WP_000938723.1 FliA/WhiG family RNA polymerase sigma factor -
EL019_RS17445 3563769..3564236 - 468 WP_000725257.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T286619 WP_001263489.1 NZ_LR134075:c3559757-3559359 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT286619 WP_000554758.1 NZ_LR134075:c3560053-3559760 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References