Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3783646..3784340 Replicon chromosome
Accession NZ_LR134031
Organism Escherichia coli strain NCTC11151

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag EL024_RS18865 Protein ID WP_001263500.1
Coordinates 3783646..3784044 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EL024_RS18870 Protein ID WP_000554758.1
Coordinates 3784047..3784340 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL024_RS18840 3779011..3779469 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
EL024_RS18845 3779730..3781187 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
EL024_RS18850 3781244..3781858 - 615 WP_000602123.1 peptide chain release factor H -
EL024_RS18855 3781855..3782994 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
EL024_RS18860 3783184..3783636 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
EL024_RS18865 3783646..3784044 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EL024_RS18870 3784047..3784340 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EL024_RS18875 3784392..3785447 - 1056 WP_001226168.1 DNA polymerase IV -
EL024_RS18880 3785518..3786303 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
EL024_RS18885 3786275..3787987 + 1713 Protein_3638 flagellar biosynthesis protein FlhA -
EL024_RS24735 3788066..3788224 + 159 WP_014639450.1 hypothetical protein -
EL024_RS18890 3788307..3788804 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3783646..3803999 20353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T286594 WP_001263500.1 NZ_LR134031:c3784044-3783646 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT286594 WP_000554758.1 NZ_LR134031:c3784340-3784047 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References