Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3553416..3554095 Replicon chromosome
Accession NZ_LR134031
Organism Escherichia coli strain NCTC11151

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag EL024_RS17785 Protein ID WP_000057524.1
Coordinates 3553793..3554095 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag EL024_RS17780 Protein ID WP_000806442.1
Coordinates 3553416..3553757 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL024_RS17770 3549660..3550592 - 933 WP_000883052.1 glutaminase A -
EL024_RS17775 3550854..3553358 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
EL024_RS17780 3553416..3553757 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
EL024_RS17785 3553793..3554095 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EL024_RS17790 3554228..3555022 + 795 WP_000365177.1 TraB/GumN family protein -
EL024_RS17795 3555226..3555705 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
EL024_RS17800 3555729..3556529 + 801 Protein_3425 hypothetical protein -
EL024_RS17805 3556526..3557029 + 504 WP_000667000.1 hypothetical protein -
EL024_RS17810 3557067..3558719 - 1653 WP_001513633.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3546105..3557029 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T286592 WP_000057524.1 NZ_LR134031:c3554095-3553793 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT286592 WP_000806442.1 NZ_LR134031:c3553757-3553416 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References