Detailed information of TA system TA028657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2378581..2379157
• Replicon: chromosome
• Accession: NC_019902
• Organism: Thioalkalivibrio nitratireducens DSM 14787

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: TVNIR_RS19120
• Protein ID: WP_083499447.1
• Coordinates: 2378879..2379157 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: TVNIR_RS10925
• Protein ID: WP_043740574.1
• Coordinates: 2378581..2378868 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TVNIR_RS19105	2374112..2374339	-	228	WP_083499445.1	type II toxin-antitoxin system HicA family toxin	-
TVNIR_RS19110	2374336..2374539	-	204	WP_083499446.1	type II toxin-antitoxin system HicB family antitoxin	-
TVNIR_RS10915	2374778..2375512	+	735	WP_015259084.1	site-specific integrase	-
TVNIR_RS10920	2375855..2378170	-	2316	WP_015259085.1	IS4 family transposase	-
TVNIR_RS10925	2378581..2378868	-	288	WP_043740574.1	HigA family addiction module antidote protein	Antitoxin
TVNIR_RS19120	2378879..2379157	-	279	WP_083499447.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TVNIR_RS19125	2379275..2379640	-	366	WP_083499448.1	glyoxalase/bleomycin resistance/extradiol dioxygenase family protein	-
TVNIR_RS10930	2380219..2381574	+	1356	WP_043739628.1	group II intron reverse transcriptase/maturase	-
TVNIR_RS19805	2381669..2382319	-	651	WP_157092262.1	hypothetical protein	-
TVNIR_RS10935	2382332..2384107	-	1776	WP_015259088.1	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2374778..2388396	13618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-86)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10304.76 Da
• Isoelectric Point: 10.9619

>T28657 WP_083499447.1 NC_019902:c2379157-2378879 [Thioalkalivibrio nitratireducens DSM 14787]
MIRSFKHKGLARFFRSGSTAGIQAAHAKRLRLILGRLNAASEIKDMDLAGLRLHKLSGGRAGTWSVTVSGNWRVTFRFED
GDAEIVNYEDYH

Nucleotide

• Length: 279 bp

>T28657 NC_019902:c2379157-2378879 [Thioalkalivibrio nitratireducens DSM 14787]
ATGATCCGAAGCTTCAAGCACAAGGGTTTGGCCAGATTTTTCAGGTCCGGTAGCACCGCAGGGATACAGGCTGCTCATGC
GAAGAGGCTTCGATTGATTCTCGGCAGGCTAAATGCAGCGTCAGAAATCAAAGACATGGACTTGGCAGGCCTTCGGCTGC
ACAAGCTCTCTGGGGGTCGCGCCGGGACTTGGTCGGTAACAGTTAGCGGTAACTGGCGAGTGACCTTCCGGTTCGAGGAC
GGAGACGCCGAGATAGTGAACTATGAAGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10448.10 Da
• Isoelectric Point: 7.3594

>AT28657 WP_043740574.1 NC_019902:c2378868-2378581 [Thioalkalivibrio nitratireducens DSM 14787]
MLMHNPPHPGEVLRELCLEPMGLSVTAAAEALGVSRKTLSSVLNGKAGISPEMAIRLSIAFDTSAESWLNQQSQYELWHA
EQHRKELKVKKLVAA

Nucleotide

• Length: 288 bp

>AT28657 NC_019902:c2378868-2378581 [Thioalkalivibrio nitratireducens DSM 14787]
ATGTTGATGCACAATCCCCCCCATCCTGGTGAAGTGTTGCGTGAGCTTTGTCTGGAGCCCATGGGTCTCTCTGTAACGGC
CGCTGCAGAAGCGCTGGGAGTAAGTCGAAAAACCCTGAGTTCCGTATTGAACGGTAAGGCCGGCATTAGTCCAGAGATGG
CCATTCGACTATCCATTGCGTTTGATACTTCGGCAGAGAGTTGGCTGAACCAGCAGTCCCAGTATGAGCTCTGGCATGCT
GAGCAGCACCGTAAAGAGCTCAAGGTTAAAAAGCTTGTCGCCGCCTGA