Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 660626..661353 Replicon chromosome
Accession NZ_LR134000
Organism Escherichia coli strain NCTC9066

Toxin (Protein)


Gene name higB Uniprot ID S1EX98
Locus tag EL014_RS03210 Protein ID WP_000550192.1
Coordinates 660626..660940 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EL014_RS03215 Protein ID WP_000560266.1
Coordinates 660937..661353 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL014_RS03190 656793..657779 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
EL014_RS03195 657858..658541 - 684 WP_001183046.1 vancomycin high temperature exclusion protein -
EL014_RS03200 658618..659121 - 504 WP_001295542.1 M48 family metallopeptidase -
EL014_RS03205 659206..660342 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
EL014_RS03210 660626..660940 + 315 WP_000550192.1 type II toxin-antitoxin system toxin HigB Toxin
EL014_RS03215 660937..661353 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
EL014_RS03220 661398..663416 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
EL014_RS03225 663842..666193 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12063.08 Da        Isoelectric Point: 10.0409

>T286560 WP_000550192.1 NZ_LR134000:660626-660940 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTPKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT286560 WP_000560266.1 NZ_LR134000:660937-661353 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EX98

Antitoxin

Source ID Structure

References