Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4868704..4869306 Replicon chromosome
Accession NZ_LR130562
Organism Escherichia coli strain MS14384 isolate MS14384

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag EW037_RS23590 Protein ID WP_000897305.1
Coordinates 4868995..4869306 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EW037_RS23585 Protein ID WP_000356395.1
Coordinates 4868704..4868994 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW037_RS23545 4864328..4865230 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
EW037_RS23550 4865227..4865862 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
EW037_RS23555 4865859..4866788 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
EW037_RS23560 4866970..4867212 - 243 WP_001309881.1 ribbon-helix-helix domain-containing protein -
EW037_RS23565 4867431..4867649 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
EW037_RS23575 4868068..4868346 - 279 WP_001315112.1 hypothetical protein -
EW037_RS23580 4868398..4868619 - 222 WP_001550354.1 hypothetical protein -
EW037_RS23585 4868704..4868994 - 291 WP_000356395.1 helix-turn-helix domain-containing protein Antitoxin
EW037_RS23590 4868995..4869306 - 312 WP_000897305.1 hypothetical protein Toxin
EW037_RS23595 4869535..4870443 + 909 WP_001550353.1 alpha/beta hydrolase -
EW037_RS23600 4870611..4872425 - 1815 WP_001550352.1 hypothetical protein -
EW037_RS23605 4872841..4873782 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
EW037_RS23610 4873827..4874264 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T286474 WP_000897305.1 NZ_LR130562:c4869306-4868995 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT286474 WP_000356395.1 NZ_LR130562:c4868994-4868704 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References