286455

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1163120..1163847 Replicon chromosome
Accession NZ_LR130562
Organism Escherichia coli strain MS14384 isolate MS14384

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag EW037_RS05625 Protein ID WP_000547555.1
Coordinates 1163120..1163431 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EW037_RS05630 Protein ID WP_000126294.1
Coordinates 1163428..1163847 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW037_RS05600 1159055..1160764 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
EW037_RS05605 1160774..1161316 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
EW037_RS05610 1161316..1162083 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
EW037_RS05615 1162080..1162490 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
EW037_RS05620 1162483..1162953 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
EW037_RS05625 1163120..1163431 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
EW037_RS05630 1163428..1163847 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
EW037_RS05635 1163926..1165350 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
EW037_RS05640 1165359..1166816 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
EW037_RS05645 1167076..1168086 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
EW037_RS05650 1168235..1168762 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T286455 WP_000547555.1 NZ_LR130562:1163120-1163431 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT286455 WP_000126294.1 NZ_LR130562:1163428-1163847 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References