Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4880561..4881163 Replicon chromosome
Accession NZ_LR130555
Organism Escherichia coli strain MS14385 isolate MS14385

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag EW040_RS23970 Protein ID WP_000897305.1
Coordinates 4880852..4881163 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EW040_RS23965 Protein ID WP_000356397.1
Coordinates 4880561..4880851 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW040_RS23940 4877063..4877965 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
EW040_RS23945 4877962..4878597 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
EW040_RS23950 4878594..4879523 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
EW040_RS23955 4879738..4879956 - 219 WP_001298592.1 ribbon-helix-helix domain-containing protein -
EW040_RS23965 4880561..4880851 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
EW040_RS23970 4880852..4881163 - 312 WP_000897305.1 hypothetical protein Toxin
EW040_RS23975 4881392..4882300 + 909 WP_001318161.1 alpha/beta hydrolase -
EW040_RS23980 4882364..4883305 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
EW040_RS23985 4883350..4883787 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
EW040_RS23990 4883784..4884656 - 873 WP_000920762.1 virulence factor BrkB family protein -
EW040_RS23995 4884650..4885249 - 600 WP_001314324.1 glucose-1-phosphatase -
EW040_RS24000 4885348..4886133 - 786 WP_000059679.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T286438 WP_000897305.1 NZ_LR130555:c4881163-4880852 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT286438 WP_000356397.1 NZ_LR130555:c4880851-4880561 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References