Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3949098..3949792 Replicon chromosome
Accession NZ_LR130555
Organism Escherichia coli strain MS14385 isolate MS14385

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag EW040_RS19335 Protein ID WP_001263491.1
Coordinates 3949098..3949496 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag EW040_RS19340 Protein ID WP_000554755.1
Coordinates 3949499..3949792 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW040_RS19305 3944360..3945817 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
EW040_RS19310 3945826..3946107 + 282 WP_022645226.1 hypothetical protein -
EW040_RS19315 3946124..3946633 - 510 WP_001361775.1 hydrolase -
EW040_RS19320 3946695..3947309 - 615 WP_022645225.1 peptide chain release factor H -
EW040_RS19325 3947306..3948445 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
EW040_RS19330 3948636..3949088 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
EW040_RS19335 3949098..3949496 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EW040_RS19340 3949499..3949792 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EW040_RS19345 3949844..3950899 - 1056 WP_022645222.1 DNA polymerase IV -
EW040_RS19350 3950970..3951755 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
EW040_RS19355 3951727..3953439 + 1713 Protein_3737 flagellar biosynthesis protein FlhA -
EW040_RS19360 3953537..3954310 - 774 WP_022645219.1 C40 family peptidase -
EW040_RS19365 3954496..3954756 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T286432 WP_001263491.1 NZ_LR130555:c3949496-3949098 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT286432 WP_000554755.1 NZ_LR130555:c3949792-3949499 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References