Detailed information of TA system TA028642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 498192..498782
• Replicon: chromosome
• Accession: NC_019902
• Organism: Thioalkalivibrio nitratireducens DSM 14787

Toxin (Protein)

• Gene name: graT
• Uniprot ID: L0DTA6
• Locus tag: TVNIR_RS02405
• Protein ID: WP_015257379.1
• Coordinates: 498504..498782 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TVNIR_RS02400
• Protein ID: WP_015257378.1
• Coordinates: 498192..498491 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TVNIR_RS02355	493367..493624	-	258	WP_043739112.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TVNIR_RS02360	494041..494421	+	381	WP_015257371.1	nucleotidyltransferase domain-containing protein	-
TVNIR_RS02365	494411..494845	+	435	WP_015257372.1	DUF86 domain-containing protein	-
TVNIR_RS02370	495035..495415	-	381	WP_015257373.1	helix-turn-helix transcriptional regulator	-
TVNIR_RS02375	495412..495669	-	258	WP_043739114.1	type II toxin-antitoxin system RelE/ParE family toxin	-
TVNIR_RS02380	495888..496151	-	264	WP_015257374.1	hypothetical protein	-
TVNIR_RS02385	496362..496793	-	432	WP_015257375.1	type II toxin-antitoxin system VapC family toxin	-
TVNIR_RS02390	496790..497029	-	240	WP_015257376.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
TVNIR_RS02395	497238..497727	-	490	Protein_480	hypothetical protein	-
TVNIR_RS02400	498192..498491	-	300	WP_015257378.1	HigA family addiction module antidote protein	Antitoxin
TVNIR_RS02405	498504..498782	-	279	WP_015257379.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TVNIR_RS02410	499004..499978	-	975	WP_015257380.1	hypothetical protein	-
TVNIR_RS02415	500561..500803	-	243	WP_043739116.1	hypothetical protein	-
TVNIR_RS02420	500803..501249	-	447	WP_211263138.1	putative toxin-antitoxin system toxin component, PIN family	-
TVNIR_RS02425	501707..502657	-	951	WP_015257383.1	hypothetical protein	-
TVNIR_RS02430	503016..503567	-	552	Protein_487	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	495888..509195	13307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10648.15 Da
• Isoelectric Point: 8.5523

>T28642 WP_015257379.1 NC_019902:c498782-498504 [Thioalkalivibrio nitratireducens DSM 14787]
MIRSFRCKDTQALFETGKTRRWPSLKAVAERKLIQLHAAEALADLKAPPGNRLEALAGDREGQHSIRINDQWRICFRWTP
EGPEHVEIVDYH

Nucleotide

• Length: 279 bp

>T28642 NC_019902:c498782-498504 [Thioalkalivibrio nitratireducens DSM 14787]
ATGATCAGGAGCTTCCGCTGCAAGGACACGCAGGCGCTGTTCGAGACAGGAAAGACCCGCCGATGGCCTTCTCTCAAGGC
GGTGGCGGAACGCAAGCTTATACAACTACATGCGGCGGAAGCCCTCGCTGATCTCAAGGCACCCCCAGGCAACCGGCTGG
AGGCGCTCGCAGGCGATCGAGAGGGCCAGCACAGCATTCGGATCAACGACCAGTGGCGAATCTGTTTCCGCTGGACGCCG
GAAGGACCCGAGCATGTAGAAATCGTGGATTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11235.89 Da
• Isoelectric Point: 10.0783

>AT28642 WP_015257378.1 NC_019902:c498491-498192 [Thioalkalivibrio nitratireducens DSM 14787]
MSTNGMRPIHPGEILREEYLAPLGMSVNALAGALHVPATRMNDIVRERRGVTADTALRLARYFGTTERYWLNLQTEYELR
LAQIQSAARVRREVEPRAA

Nucleotide

• Length: 300 bp

>AT28642 NC_019902:c498491-498192 [Thioalkalivibrio nitratireducens DSM 14787]
ATGAGTACCAACGGCATGCGCCCTATCCATCCCGGCGAGATCCTCCGCGAGGAGTACCTGGCGCCCCTGGGCATGAGCGT
GAATGCCTTGGCGGGCGCGCTGCACGTGCCGGCGACCCGCATGAATGACATCGTTCGCGAGCGCCGCGGCGTAACGGCGG
ACACCGCCCTGAGACTCGCACGCTATTTTGGTACCACTGAGCGCTACTGGCTGAACCTGCAAACCGAATACGAACTTCGC
CTCGCGCAGATCCAGTCTGCCGCCCGCGTACGCCGCGAGGTCGAACCGCGCGCGGCTTAG

Structures

Toxin

• AlphaFold DB: L0DTA6