Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4640718..4641320 Replicon chromosome
Accession NZ_LR130552
Organism Escherichia coli strain MS14386 isolate MS14386

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag EW035_RS22430 Protein ID WP_000897305.1
Coordinates 4641009..4641320 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EW035_RS22425 Protein ID WP_000356397.1
Coordinates 4640718..4641008 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW035_RS22400 4636663..4637565 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
EW035_RS22405 4637562..4638197 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
EW035_RS22410 4638194..4639123 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
EW035_RS22415 4639453..4639695 - 243 WP_001086388.1 hypothetical protein -
EW035_RS22420 4639914..4640132 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
EW035_RS22425 4640718..4641008 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
EW035_RS22430 4641009..4641320 - 312 WP_000897305.1 hypothetical protein Toxin
EW035_RS22435 4641549..4642457 + 909 WP_001162704.1 alpha/beta hydrolase -
EW035_RS22440 4642521..4643462 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
EW035_RS22445 4643507..4643944 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
EW035_RS22450 4643941..4644813 - 873 WP_000920762.1 virulence factor BrkB family protein -
EW035_RS22455 4644807..4645406 - 600 WP_001295269.1 glucose-1-phosphatase -
EW035_RS22460 4645505..4646290 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T286411 WP_000897305.1 NZ_LR130552:c4641320-4641009 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT286411 WP_000356397.1 NZ_LR130552:c4641008-4640718 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References