Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3765331..3765872 Replicon chromosome
Accession NZ_LR130552
Organism Escherichia coli strain MS14386 isolate MS14386

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EW035_RS18260 Protein ID WP_000615976.1
Coordinates 3765594..3765872 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EW035_RS18255 Protein ID WP_000729704.1
Coordinates 3765331..3765591 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW035_RS18230 3760367..3761506 + 1140 WP_023155855.1 flagellar type III secretion system protein FlhB -
EW035_RS18235 3761490..3763583 + 2094 WP_023155854.1 flagellar biosynthesis protein FlhA -
EW035_RS18240 3763688..3763966 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EW035_RS18245 3763959..3764315 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EW035_RS18250 3764372..3765145 - 774 WP_000543899.1 C40 family peptidase -
EW035_RS18255 3765331..3765591 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EW035_RS18260 3765594..3765872 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EW035_RS18265 3766028..3766768 + 741 WP_001225679.1 murein L,D-transpeptidase -
EW035_RS18270 3766739..3767506 - 768 WP_000333380.1 class II glutamine amidotransferase -
EW035_RS18275 3767712..3768290 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T286407 WP_000615976.1 NZ_LR130552:3765594-3765872 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT286407 WP_000729704.1 NZ_LR130552:3765331-3765591 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References