Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 622941..623740 Replicon chromosome
Accession NZ_LR130552
Organism Escherichia coli strain MS14386 isolate MS14386

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag EW035_RS03035 Protein ID WP_000347267.1
Coordinates 622941..623405 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EW035_RS03040 Protein ID WP_001307405.1
Coordinates 623405..623740 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW035_RS03005 617942..618376 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
EW035_RS03010 618394..619272 - 879 WP_001298758.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
EW035_RS03015 619262..620041 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
EW035_RS03020 620052..620525 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EW035_RS03025 620548..621828 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
EW035_RS03030 622077..622886 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
EW035_RS03035 622941..623405 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EW035_RS03040 623405..623740 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EW035_RS03045 623888..625459 - 1572 WP_001273741.1 galactarate dehydratase -
EW035_RS03050 625834..627168 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
EW035_RS03055 627184..627954 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T286395 WP_000347267.1 NZ_LR130552:c623405-622941 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT286395 WP_001307405.1 NZ_LR130552:c623740-623405 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References