Detailed information of TA system TA286374

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20177..20603
• Replicon: plasmid 2
• Accession: NZ_LR130546
• Organism: Escherichia coli strain B36 isolate B36

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EW036_RS25880
• Protein ID: WP_001312861.1
• Coordinates: 20445..20603 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20177..20401 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW036_RS25855	16169..16654	+	486	WP_042029316.1	single-stranded DNA-binding protein	-
EW036_RS25860	16710..16943	+	234	WP_000006004.1	DUF905 domain-containing protein	-
EW036_RS25865	17002..18960	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
EW036_RS25870	19015..19449	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
EW036_RS25875	19446..20165	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
EW036_RS26685	20177..20365	-	189	WP_001299721.1	hypothetical protein	-
-	20177..20401	+	225	NuclAT_0	-	Antitoxin
-	20177..20401	+	225	NuclAT_0	-	Antitoxin
-	20177..20401	+	225	NuclAT_0	-	Antitoxin
-	20177..20401	+	225	NuclAT_0	-	Antitoxin
EW036_RS25880	20445..20603	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
EW036_RS25905	21522..21809	+	288	WP_000107537.1	hypothetical protein	-
EW036_RS25910	21930..22751	+	822	WP_001234469.1	DUF945 domain-containing protein	-
EW036_RS25915	23048..23695	-	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
EW036_RS25920	23981..24364	+	384	WP_001151564.1	relaxosome protein TraM	-
EW036_RS25925	24558..25244	+	687	WP_000332487.1	PAS domain-containing protein	-
EW036_RS25930	25338..25565	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	-	1..112588	112588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T286374 WP_001312861.1 NZ_LR130546:20445-20603 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT286374 NZ_LR130546:20177-20401 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin