Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3943795..3944392 Replicon chromosome
Accession NZ_LR130543
Organism Klebsiella variicola strain 04153260899A isolate 04153260899A

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2LYJ8
Locus tag EW039_RS19540 Protein ID WP_023322191.1
Coordinates 3944075..3944392 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6H0BIE0
Locus tag EW039_RS19535 Protein ID WP_023322192.1
Coordinates 3943795..3944082 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW039_RS19505 3939761..3940009 + 249 WP_008805539.1 DUF1158 domain-containing protein -
EW039_RS19510 3940026..3940368 - 343 Protein_3760 RamA family antibiotic efflux transcriptional regulator -
EW039_RS19515 3940399..3941514 - 1116 WP_172601108.1 MBL fold metallo-hydrolase -
EW039_RS19520 3941694..3942218 + 525 WP_129678742.1 TetR/AcrR family transcriptional regulator -
EW039_RS19525 3942218..3942586 + 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
EW039_RS19530 3942706..3943359 + 654 WP_008805535.1 oxygen-insensitive NAD(P)H nitroreductase -
EW039_RS19535 3943795..3944082 - 288 WP_023322192.1 helix-turn-helix transcriptional regulator Antitoxin
EW039_RS19540 3944075..3944392 - 318 WP_023322191.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EW039_RS19545 3944566..3945621 - 1056 WP_023322190.1 DUF2157 domain-containing protein -
EW039_RS19550 3946284..3947150 - 867 WP_008805530.1 helix-turn-helix transcriptional regulator -
EW039_RS19555 3947259..3948686 + 1428 WP_023322189.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-89)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T286328 WP_023322191.1 NZ_LR130543:c3944392-3944075 [Klebsiella variicola]
MFRIVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10801.48 Da        Isoelectric Point: 10.2212

>AT286328 WP_023322192.1 NZ_LR130543:c3944082-3943795 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANVTRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H0BIE0

References