Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 5786048..5786823 Replicon chromosome
Accession NZ_LR130533
Organism Pseudomonas aeruginosa isolate paerg009

Toxin (Protein)


Gene name yhaV Uniprot ID V6ADY6
Locus tag EJN60_RS27855 Protein ID WP_009518525.1
Coordinates 5786365..5786823 (+) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A1L1PS41
Locus tag EJN60_RS27850 Protein ID WP_009518526.1
Coordinates 5786048..5786365 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJN60_RS27840 5781182..5783833 + 2652 WP_125075549.1 excinuclease ABC subunit UvrA -
EJN60_RS27845 5783911..5785749 - 1839 WP_125075550.1 TraI domain-containing protein -
EJN60_RS27850 5786048..5786365 + 318 WP_009518526.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
EJN60_RS27855 5786365..5786823 + 459 WP_009518525.1 type II toxin-antitoxin system YhaV family toxin Toxin
EJN60_RS27860 5786850..5787227 + 378 WP_009518524.1 DUF3742 family protein -
EJN60_RS27865 5787243..5788760 - 1518 WP_009518523.1 conjugal transfer protein TraG N-terminal domain-containing protein -
EJN60_RS27870 5788775..5789134 - 360 WP_009518522.1 hypothetical protein -
EJN60_RS27875 5789131..5790525 - 1395 WP_009518521.1 integrating conjugative element protein -
EJN60_RS27880 5790535..5791482 - 948 WP_009518520.1 TIGR03756 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 5758264..5844582 86318


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-100)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17660.02 Da        Isoelectric Point: 10.1848

>T286246 WP_009518525.1 NZ_LR130533:5786365-5786823 [Pseudomonas aeruginosa]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11156.69 Da        Isoelectric Point: 4.7182

>AT286246 WP_009518526.1 NZ_LR130533:5786048-5786365 [Pseudomonas aeruginosa]
MPAIHEVATLTSKGQVTLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNVRGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ADY6

Antitoxin

Source ID Structure
AlphaFold DB A0A1L1PS41

References