Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2248366..2248961 Replicon chromosome
Accession NZ_LR130533
Organism Pseudomonas aeruginosa isolate paerg009

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag EJN60_RS11050 Protein ID WP_003113526.1
Coordinates 2248683..2248961 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag EJN60_RS11045 Protein ID WP_003111575.1
Coordinates 2248366..2248671 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJN60_RS11010 2243506..2244354 + 849 WP_023096155.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
EJN60_RS11020 2244521..2245462 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
EJN60_RS11025 2245579..2246193 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
EJN60_RS11030 2246235..2246819 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
EJN60_RS11035 2246860..2247960 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
EJN60_RS11045 2248366..2248671 - 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
EJN60_RS11050 2248683..2248961 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EJN60_RS11060 2249290..2251518 + 2229 WP_021264080.1 TonB-dependent receptor -
EJN60_RS11065 2251588..2252235 - 648 WP_003095021.1 carbonate dehydratase -
EJN60_RS11070 2252297..2253535 - 1239 WP_014603324.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T286242 WP_003113526.1 NZ_LR130533:c2248961-2248683 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT286242 WP_003111575.1 NZ_LR130533:c2248671-2248366 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References