Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4205987..4206786 Replicon chromosome
Accession NZ_LR130532
Organism Escherichia coli isolate ecoli019

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag EJK95_RS20570 Protein ID WP_000347267.1
Coordinates 4206322..4206786 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EJK95_RS20565 Protein ID WP_001307405.1
Coordinates 4205987..4206322 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJK95_RS20550 4201774..4202544 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
EJK95_RS20555 4202560..4203858 - 1299 WP_000557469.1 galactarate/glucarate/glycerate transporter GarP -
EJK95_RS20560 4204267..4205838 + 1572 WP_001273741.1 galactarate dehydratase -
EJK95_RS20565 4205987..4206322 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EJK95_RS20570 4206322..4206786 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EJK95_RS20575 4206841..4207650 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
EJK95_RS20580 4207899..4209179 + 1281 WP_106474742.1 tagatose-bisphosphate aldolase subunit KbaZ -
EJK95_RS20585 4209202..4209675 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EJK95_RS20590 4209686..4210465 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
EJK95_RS20595 4210455..4211333 + 879 WP_001355782.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
EJK95_RS20600 4211351..4211785 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4195571..4206786 11215


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T286236 WP_000347267.1 NZ_LR130532:4206322-4206786 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT286236 WP_001307405.1 NZ_LR130532:4205987-4206322 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References