Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 106543..107145 Replicon chromosome
Accession NZ_LR130532
Organism Escherichia coli isolate ecoli019

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag EJK95_RS00520 Protein ID WP_000897305.1
Coordinates 106543..106854 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EJK95_RS00525 Protein ID WP_000356397.1
Coordinates 106855..107145 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJK95_RS00490 101573..102358 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
EJK95_RS00495 102457..103056 + 600 WP_001295269.1 glucose-1-phosphatase -
EJK95_RS00500 103050..103922 + 873 WP_000920762.1 virulence factor BrkB family protein -
EJK95_RS00505 103919..104356 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
EJK95_RS00510 104401..105342 + 942 WP_001343389.1 fatty acid biosynthesis protein FabY -
EJK95_RS00515 105406..106314 - 909 WP_106474777.1 alpha/beta hydrolase -
EJK95_RS00520 106543..106854 + 312 WP_000897305.1 hypothetical protein Toxin
EJK95_RS00525 106855..107145 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
EJK95_RS00530 107750..107968 + 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
EJK95_RS00535 108187..108429 + 243 WP_001086388.1 hypothetical protein -
EJK95_RS00540 108759..109688 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
EJK95_RS00545 109685..110320 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
EJK95_RS00550 110317..111219 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T286213 WP_000897305.1 NZ_LR130532:106543-106854 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT286213 WP_000356397.1 NZ_LR130532:106855-107145 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References